METHODS TO MODULATE THE ACTIVITY OF THE OESTROGEN RECEPTOR

摘要

A polypeptide having the amino acid sequence of Seq ID No 1; Figure 1A or an amino acid sequence having at least 45 or 50 % identity with the amino acid sequence of Seq ID No 1 for use in medicine. Use of a compound for modulating, for example a nuclear receptor DNA binding protein, wherein the compound is: (a) the polypeptide ERZFP (SEQ ID No 1; Figure 1A); or, (b) a fragment of the polypeptide ERZPF which binds to the transcription factor, for example a nuclear receptor DNA binding protein; or, (c) a variant of the polypeptide or fragment as defined in (a) or (b) which binds to the transcription factor, for example a nuclear receptor DNA binding protein; or (d) a fusion or derivative of the polypeptide or fragment as defined in (a), (b) or (c) which binds to the transcription factor, for example a nuclear receptor DNA binding protein; or (e) a peptidomimetic of the polypeptide, fragment, variant, fusion or derivative as defined in (a), (b), (c), or (d) which binds to the transcription factor, for example a nuclear receptor DNA binding protein; or, (f) a compound, for example an antibody or antibody fragment which mimics the binding of the polypeptide, fragment, variant, fusion or derivative as defined in (a), (b), (c), or (d) to the transcription factor, for example a nuclear receptor DNA binding protein. A method for identifying a compound which modulates, for example promotes the transcription factor activity of a transcription factor, for example a nuclear receptor DNA binding protein, comprising the steps of: (a) providing the transcription factor, for example a nuclear receptor DNA binding protein or a fragment thereof comprising the AF1 domain for fragment thereof, and a compound as defined above; (b) exposing a test compound to the transcription factor, for example a nuclear receptor DNA binding protein or fragment and/or a compound as defined above; (c) determining whether the test compound modulates, for example inhibits, the ability of the transcription factor, for example a nuclear receptor DNA binding protein or fragment to bind to the compound as defined above; (d) selecting a compound which modulates, for example enhibits, the ability of the transcription factor, for example a nuclear receptor DNA binding protein or fragment to bind to the compound as defined above.