| 摘要 |
<p>A nucleic acid amplifier capable of efficiently performing PCR even with the use of nucleic acid synthetase lacking heat resistance, which nucleic acid amplifier can realize nucleic acid synthetase reutilization, scaleup, etc. and facilitates separation/purification of nucleic acid after amplification; and a method of nucleic acid amplification performed therewith. In particular, the method comprises causing at least a nucleic acid as a template, a nucleic acid as a primer and a reaction solution containing a phosphate compound and a metal ion to flow through a channel having a region for melting an intramolecularly and/or intermolecularly formed double strand of nucleic acid so as to effect denaturation into single strands and a regeneration region for regenerating a double strand of nucleic acid from the nucleic acid after the double strand melting, thereby melting at the denaturation region the intramolecularly and/or intermolecularly formed double strand of nucleic acid as the template so as to effect denaturation into single strands and thereafter at the regeneration region not only regenerating a double strand between the template nucleic acid after the double strand melting and the primer nucleic acid but also carrying out nucleic acid synthesis with the nucleic acid synthetase fixed within the regeneration region.</p> |
