METHOD FOR ISOLATION AND SELECTION OF MICROORGANISMS AS PRODUCERS OF CYCLODEXTRIN GLUCANOTRASNFERASE, STRAIN OF MICROORGANISM BACILLUS CIRCULANS B-65 NCAIM (P) 001277 (B-65) AS PRODUCER OF EXTRACELLULAR CYCLODEXTRIN TRANSFERASE, CYCLODEXTRIN GLUCANOTRANSFERASE OBTAINED FROM ITS AND ITS APPLYING FOR PREPARING CYCLODEXTRIN

摘要

FIELD: biotechnology, biochemistry, microbiology. ^ SUBSTANCE: the strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of beta-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry. ^ EFFECT: improved isolating method, valuable properties of strain and enzymes. ^ 9 cl, 8 tbl, 2 ex