PYROPHOSPHORIC ACID DETECTION SENSOR, METHOD OF DETECTING NUCLEIC ACID AND METHOD OF DISCRIMINATING BASE VARIETY

摘要

A pyrophosphoric acid detection sensor for detecting pyrophosphoric acid in a sample liquid, which comprises a sample liquid reception part for receiving the sample liquid; an H<+> permeation restricting membrane containing H<+> pyrophosphatase; an immobilization layer for fixing the H<+> permeation restricting membrane; and measuring means for measuring an electrochemical change accompanying a change of hydrogen ion concentration in the immobilization layer. The above H<+> pyrophosphatase is arranged so that it effects hydrolysis of any pyrophosphoric acid in the sample liquid, thereby inducing a change in the hydrogen ion concentration in the immobilization layer.