发明名称 |
Enzymatic production of difructose dianhydride IV from sucrose and relevant enzymes and genes coding for them |
摘要 |
Disclosed is the production of difructose dianhydride IV from sugar. A sugar solution is subjected to reaction at room temperature or lower in an acidic buffer of pH 3.0-7.0 in the presence of a levansucrase derived from Z. mobilis to produce levan. The levansucrase is prepared by culturing E. coli BL21 (DE3)/pEL12 (KCTC 8661), harvesting and homogenizing the cells, and isolating levansucrase from the cell homogenate. Levan is purified from the reaction solution and subjected to reaction at 25-50° C. for 3-10 hours in an acidic buffer of pH 3.0-7.0 in the presence of a levan fructotransferase to produce difructose dianhydride IV. The levan fructotransferase is obtained from E. coli JUD81 (KCTC 0877BP). Also, disclosed are a gene coding for the levan fructotransferase and an expression vector pUDAF81 carrying the gene.
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申请公布号 |
US6841368(B1) |
申请公布日期 |
2005.01.11 |
申请号 |
US20010868328 |
申请日期 |
2001.11.09 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY;REALBIOTECH LTD. |
发明人 |
RHEE SANGKI;SONG KIBANG;KIM CHULHO;RYU EUNJA;LEE YONGBOK |
分类号 |
C12N15/09;C12N1/20;C12N1/21;C12N9/10;C12P19/18;C12R1/06;(IPC1-7):C12N9/00;C12N9/12;C12N15/00;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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