UTILIZATION OF CELL FORCIBLY EXPRESSING TOLL-LIKE RECEPTOR

摘要

A cell forcibly expressing swine Toll-like receptor 9 (TLR9) is prepared by cloning a TLR9 gene from swine intestinal tract Peyer's patches. As the results of functional analysis on CpG DNAS using the above cell, it is clarified that swine TLR9 shows higher recognizability for a human CpG DNA motif (CpG2006) than for a mouse-specific CpG DNA motif (CpG1826). When mRNA expression amounts in various tissues are compared by the Real-time PCR method, it is found out that mRNA is expressed in Peyer's patches and intestinal tract membrane lymph nods, which play important roles in the intestinal tract immune system, in an amount thrice or more as much as in spleen. Thus, the cell forcibly expressing TLR, which is expressed in intestinal tract tissues (for example, TLR9), is usable in identifying a sample capable of activating the intestinal tract immune system.