The invention provides methods and diagnostic test kits for detecting target nucleic acid sequence variations in a sample. In particular, a plurality of oligonucleotide probe sets is provided. Each set has a target specific portion and a barcode. The target specific portions of the probes are suitable for ligation together when hybridized adjacent to one another on a corresponding target polynucleotide. The ligated product contains a barcode that binds to a probe attached to a substrate and hence facilitate the capture of the ligated product on the solid support. Detection is carried out with a gold nanoparticle-attached barcode that hybridizes with the barcode on the ligated product.
申请公布号
WO2005001113(A2)
申请公布日期
2005.01.06
申请号
WO2004US20464
申请日期
2004.06.25
申请人
THOMAS JEFFERSON UNIVERSITY;THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA;DELGROSSO, KATHLEEN;FORTINA, PAOLO;GRAVES, DAVID;SURREY, SAUL