| 摘要 |
A method of connecting an aseptic fitting to an opening in the top of an aseptic bulk storage tank, whereby asepsis of the tank interior is maintained. The method includes the step of filling the tank interior with liquid chemical sterilant sufficiently to overflow sterilant from the tank into a liquid-retaining enclosure which surrounds the opening in the tank top. This is effective to both sterilize the interior of the tank, and provide a liquid sterilant bath in contact with the openings. The method further includes immersing the fitting in the sterilant bath to sterilize it, and while immersed and in a sterile condition, connecting it to the tank opening, thereby isolating the sterile tank interior from the environment with a sterile fitting. If the fitting includes an internal cavity, sterilization of the fitting prior to its immersion in the bath and connection to the tank can be done by capping the fitting to seal the cavity and autoclaving it. The capped fitting which has been sterilized remote from the sterilant bath is then immersed in the sterilant bath and while immersed connected to the tank opening, to thereby establish communication between the cavity of the fitting and the tank interior while both the cavity and the interior are maintained in a sterile condition. |
