METHOD FOR PRODUCTION OF OLIGOSACCHARIDE FROM LAMINARIA JAPONICA BY ADDING THE CULTURED MEDIUM OF ESCHERICHIA COLI TRANSFORMED WITH VECTOR PLK108S(KFCC-11303) OR ENDO-BETA-1,3-GLUCANASE PRODUCED THEREFROM INTO LAMINARIN ISOLATED FROM LAMINARIA JAPONICA

摘要

PURPOSE: A method for production of oligosaccharide from Laminaria japonica is provided, thereby rapidly and high efficiently producing oligosaccharide from Laminaria japonica, which oligosaccharide is useful for food and medicine and food industry. CONSTITUTION: The method for production of oligosaccharide from Laminaria japonica comprises the steps of: culturing Escherichia coli transformed with a vector pLK108S(KFCC-11303) in a medium; recovering endo-beta-1,3-glucanase from the supernatant of the cultured medium; extracting dried Laminaria japonica with hot water; adding ethanol into the extract of Laminaria japonica and centrifuging it at 5000 rpm for 20 minutes; sequentially filtrating the supernatant of the centrifuged culture medium with 0.1-m2 membrane, 100kD membrane and 1kD membrane to obtain the filtered active fraction; subjecting the active fraction to gel filtration to isolate laminarin; and adding the cultured medium of Escherichia coli transformed with a vector pLK108S(KFCC-11303) or endo-beta-1,3-glucanase produced therefrom into the laminarin isolated from Laminaria japonica, wherein the oligosaccharide is laminaribose or laminaripentaose.