摘要 |
A transposable element comprising at least three repeat sequences, at least one of which is inverted in relation to the others, and where at least one non-terminal repeat is a minimal repeat. DNA to be inserted into a host genome may be located between the minimal repeat and a repeat having the same orientation as the minimal repeat so that when the minimal repeat recombines a terminal repeat the inserted DNA is unable to excise. A transposable element comprising at least four inverted repeat sequences, at least two of which are inverted in relation to the others, comprising DNA for insertion into a host genome located between two pairs of opposing repeats excisable by a transposase to leave the DNA in the host genome without flanking transposon derived repeats. A method of transforming an organism, especially an insect and the organism itself are claimed. The transposon may be piggyBac. |