ENZYME/CHEMICAL REACTOR BASED PROTEIN PROCESSING METHOD FOR PROTEOMICS ANALYSIS BY MASS SPECTROMETRY
摘要
<p>A method and tools for the biochemical processing of protein samples towards their analysis and identification by, for example, mass spectrometry (MS). The method involves the reversible (non-covalent) immobilization, purification and concentration of proteins onto a solid surface and subsequent solid-phase enzyme-catalyzed proteolysis of the proteins. The constituent peptides are recovered in near-quantitative yields in a format ideally suited for identification by routine MS technologies. The process is easily adapted such that additional chemical and/or enzymatic transformation(s) of the immobilized proteins or peptides can be performed to facilitate the acquisition of and increase the information content obtained from the MS analysis.</p>
申请公布号
WO2004113923(A1)
申请公布日期
2004.12.29
申请号
WO2004CA00937
申请日期
2004.06.23
申请人
MDS PROTEOMICS INC.;DUEWEL, HENRY;GOH, THEO;LE BIHAN, THIERRY;FIGEYS, JOSEPH MICHEL DANIEL
发明人
DUEWEL, HENRY;GOH, THEO;LE BIHAN, THIERRY;FIGEYS, JOSEPH MICHEL DANIEL