发明名称 |
NOVEL CARBONYL REDUCTASE, GENE ENCODING IT AND PROCESS FOR PRODUCING OPTICALLY ACTIVE ALCOHOLS USING THE SAME |
摘要 |
A polypeptide comprises: (1) an amino acid sequence (i) with 251 amino acids; or (2) an amino acid sequence based on the sequence (i) but with some amino acids deleted, added or substituted and having carbonyl reductase activity. Independent claims are also included for: (1) a DNA containing: (a) a base sequence encoding a polypeptide with an amino acid sequence (i); (b) a base sequence encoding a variant of the polypeptide; (c) a base sequence (ii) with 753 base pairs; (d) a base sequence derived from the sequence (ii) but with some bases deleted, added or substituted and encoding a polypeptide with carbonyl reductase activity; and (e) a base sequence hybridizable with the base sequence (ii), its complementary sequence or a part of them under stringent conditions and encoding a polypeptide with carbonyl reductase activity; (2) a recombinant DNA obtained by integrating the DNA into a vector; (3) transformant sustaining the recombinant DNA, or obtained by integrating the DNA into a chromosomal DNA; and (4) a process for producing a compound of formula (IV) by culturing a carbonyl compound of formula (I), (II) or (III) with the transformant cells, their culture or processed material to action on the carbonyl compound for asymmetric reduction. [Image] R : H, alkyl or aryl. |
申请公布号 |
EP1491633(A1) |
申请公布日期 |
2004.12.29 |
申请号 |
EP20030712750 |
申请日期 |
2003.03.18 |
申请人 |
MITSUBISHI CHEMICAL CORPORATION;NISSAN CHEMICAL INDUSTRIES, LTD. |
发明人 |
HIRAOKA, HIROTOSHI;UEDA, MAKOTO;HARA, MARI |
分类号 |
C12N1/21;C12N9/04;C12N15/53;C12P17/12 |
主分类号 |
C12N1/21 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|