Biosensor and method of preparation

摘要

Manufacturing a device involves irradiating a silicon substrate in the presence of oxygen; reacting at least part of the silicon substrate with a first composition; removing the layer of silicon oxide; and reacting at least one target portion with a further composition. Manufacturing (M1) a device involves (a) providing a silicon substrate having a background portion and at least one target portion having silicon-hydrogen bonds on the surface; (b) irradiating the target portions using a patterned beam of radiation in the presence of oxygen to provide a layer of silicon oxide; (c) reacting at least a part of the background portion with a first composition (C1) comprising at least one compound selected from 1-alkenes or 1-alkynes; (d) removing the layer of silicon oxide from the target portion(s); and (e) reacting at least one target portion with a further composition (C2) comprising at least one compound selected from 1-alkenes or 1-alkynes, to covalently attach the at least one compounds to the target portion(s). Independent claims are also included for: (1) a device comprising a silicon substrate having a background portion and at least one target portion having a part of a transistor structure in its surface and an organic monolayer. The organic layer is directly coupled to at least a part of the surface of the silicon substrate by covalent bonds. The organic monolayer comprises receptor compounds capable of interacting with a chemical or biological substance, in area(s) which cover the or each target portion having a part of a transistor structure. The part of the transistor structure combined with the organic monolayer containing receptor compounds forms a field effect transistor; and (2) coupling an oligosaccharide or oligopeptide derivative to a silicon surface involving reacting an alkene or an alkyne of formula oligo-O-(CH 2) m-X'-(CH 2) n-CH=CH 2or oligo-O-(CH 2) m-X'-(CH 2) n-Ctriple boundCH with a silicon substrate having Si-H bonds at its surface substantially in the absence of oxygen and in the presence of heat or UV or visible radiation. oligo : oligosaccharide or oligopeptide derivative; n and m : 1 - 36; and X' : bond, O, S, C(O)O, OC(O) or 2-4C alkenylene or alkynylene (containing one or two double and/or triple bonds).