| 摘要 |
PURPOSE: Provided are a novel alkaline lipase, a novel bacillus sp. strain producing the same, and a method of producing the lipase massively. The non-toxicity bacillus sp. strain is capable of being used in food industry, and the lipase is used for quantitative determination of lipid content. CONSTITUTION: The novel alkaline lipase shows thermostability at 40 deg.C and has an optimum temperature of activity at 35 deg.C. Further, it has no changes with calcium concentrations, and has an optimum activity at pH 8.5. Its amino acid sequence is represented by the SEQ ID NO:1. The novel bacillus sp. strain, bacillus pumilus B26 strain(KTCC 10081BP) produces the lipase. The lipase is mass-produced by transforming bacillus subtilis DB104 with a recombinant vector pMB26 then culturing the transformant. The transformant strain is bacillus subtilis DB104/pMB26(KCTC 10082BP).
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