发明名称 |
MOUSE HAVING HUMAN CYTOCHROME P450 TRANSFERRED THEREIN |
摘要 |
A partial fragment of chromosome 7 derived from human normal fibroblast is introduced into a mouse ES cell (embryonic stem cell) by a microcell method, using the ES cell, a chimeric mouse is obtained which harbors human chromosome fragment in a normal tissue, and which expresses human CYP3A4 gene in liver and small intestine due to induction by a drug. Also, to disrupt mouse endogenous P450 genes, a physical map relating to Cyp3a genes on mouse chromosome 5 is prepared, and also vectors necessary for gene targeting are prepared based on the physical map. By using the vectors, a mouse that has human P450 genes (CYP3A family) and in which mouse endogenous P450 genes (Cyp3a family) have been disrupted is prepared. |
申请公布号 |
EP1206906(A4) |
申请公布日期 |
2004.12.29 |
申请号 |
EP20000951968 |
申请日期 |
2000.08.11 |
申请人 |
KIRIN BEER KABUSHIKI KAISHA |
发明人 |
ISHIDA, ISAO;TOMIZUKA, KAZUMA;KUROIWA, YOSHIMI;OHSHIMA, TAKESHI;SUZUKI, MUTSUMI;ITOH, KUNIO |
分类号 |
A61K49/00;C12N9/02;C12N15/85;C12Q1/68;(IPC1-7):A01K67/027;G01N33/15;C12N5/10;G01N33/50;C12N15/00 |
主分类号 |
A61K49/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|