PROCESS FOR IMPROVING EFFICIENCY OF DNA AMPLIFICATION REACTION

摘要

The present Invention has an object of providing a process for improving t he efficiency of a DNA amplification reaction, and a process for improving the hybridization specificity of an oligonucleotide to a DNA. The present invention provides a process for improving the efficiency of a D NA amplification reaction, wherein a primer in which a compound such as LC-Red 705 or an oligonucleotide with a GC content of at least 25% and with at least four bas es is added to the 5' terminus is used as the primer; as well as a process for improving th e hybridization specificity of an oligonucleotide to a DNA sample, wherein an oligonucleotid e in which a compound such as LC-Red 705 is conjugated to the 5' terminus is used for hybridizing to a DNA.