PROCESS FOR PRODUCING ANTIBODY

摘要

Paying attention to the fact that no secretion is made by one H-chain due to "knobs-into-holes", it is found out that a target bispecific antibody can be preferentially formed by first expressing the H- and L-chains in one side, then suppressing the expression thereof, expressing the H- and L-chains in the other side to thereby construct target HL molecules (HaLa and HbLb) and then pairing the H chains with each other (H2L2).