COMPATIBLE HOST/VECTOR SYSTEMS FOR EXPRESSION OF DNA

摘要

The present invention provides shuttle vectors compatible with a panel of different bacterial hosts and capable of replicating in evolutionarily divergent bacterial species. In various embodiments the shuttle vectors of the present invention can integrate into the genomes of and/or replicate in both Gram-negative and Gram-positive organisms of both high and low GC content. The vectors of the present invention therefore make possible the expression of environmental DNA in varied families of bacterial hosts using a single vector that is transferable between hosts by a variety of methods. In some embodiments, the vector can replicate in a genera of bacteria from each of the following groups: Escherichia or Klebsiella; Pseudomonas, Xanthomonas, or Acetobacter; Bacillus or Clostridia, and Corynebacteria, Streptomycete, or Rhodococcus. In various embodiments, the vectors are cosmid, plasmids, fosmids, or bacterial artificial chromosomes (BACs).