| 摘要 |
In a method and system and machine-readable medium, colors in an image are unmixed using a matrix X = AS, where A is a ns. x nd matrix of spectral definitions, where s is the number of spectral components and d is the number of dyes into which the image is decomposed, and where S is a ns x / matrix of amounts of each dye at every pixel, where / is the number of pixels; the matrix X is constrained for solution by an unsupervised matrix decomposition method having constraints consistent with properties of an additive color model; and nd is determined. A texture is determined to identify areas of interest. Further processing may automatically measure cell dye concentration in the determined regions of interest. |
