发明名称 |
PRODUCTION OF HYBRIDOMA PRODUCING ANTIHUMAN CENP-A PEPTIDE MONOCLONAL ANTIBODY AND METHOD OF USING THE SAME |
摘要 |
<p>It is intended to provide antibodies, in particular, monoclonal antibodies against, as antigens, peptides comprising N-terminal 1/3 amino acid sequences of a protein CENP-A expressed in centromeres in chromosome and a process for producing the same. Antibodies against partial-length peptides of the CENP-A protein expressed in centromeres in chromosome (preferably a peptide having the amino acid sequence of 3-to 19-positions of the CENP-A protein, a peptide having the amino acid sequence of 27-to 40-positions thereof or a peptide having the amino acid sequence of 126- to 140-positions thereof); and a process for producing the same. A method of solubilizing a centromere chromatin while sustaining its completeness by treating with 0.3 M NaCl and MNase. A method of using an anti-CENP-A antibody which comprises chemically bonding the antibody to a support and isolating and purifying the same by the immunoprecipitation method.</p> |
申请公布号 |
EP1489098(A1) |
申请公布日期 |
2004.12.22 |
申请号 |
EP20020743822 |
申请日期 |
2002.07.04 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
YODA, KINYA;NOZAKI, NAOHITO;OKAZAKI, TSUNEKO |
分类号 |
C07K16/18;C12N5/20;C12P21/08;(IPC1-7):C07K16/18 |
主分类号 |
C07K16/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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