发明名称 |
Method of affinity purifying proteins using modified bis-arsenical fluorescein |
摘要 |
The present invention features methods for purifying polypeptides of interest using a modified Fluorescein arsenical helix binder (FlAsH) compound immobilized on a solid support. An exemplary FlAsH target sequence motif is also presented. Examples of modification of the FlAsH compound which allow immobilization to a solid support are also provided. The present invention also provides DNA constructs for producing a dual affinity tagged polypeptide and methods for purification thereof.
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申请公布号 |
US6831160(B1) |
申请公布日期 |
2004.12.14 |
申请号 |
US20000502664 |
申请日期 |
2000.02.11 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
VALE RONALD D.;THORN KURT;COOKE ROGER;MATSUKA MARIJA;NABER NARIMAN |
分类号 |
C07F9/80;C07K1/22;C07K19/00;(IPC1-7):A23J1/00 |
主分类号 |
C07F9/80 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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