| 摘要 |
<p>Methods for the detection and analysis of specific analytes, using techniques sensitive to surface excess are described. Detection of analytes such as nucleic acids and other biopolymers is achieved through template independent polymerization, or selective degradation. Using analyte specific initiation sites for template independent polymerisation and the measurement thereof by means of a surface sensitive technique, a functional signal amplification method is realised. When combined with immobilised specific affinity sites for the analyte, detailed information with respect to the interaction between analyte and the specific affinity sites may be obtained. Applications chiefly focus on the detection of nucleic acid based reactions and the use of the methods described to characterise nucleic acid analytes. In addition the present invention contains methods for the real time monitoring of nucleic acid substrate based enzymatic activity at the surface. Examples using voltanimetric and impedimetric techniques are described.</p> |
