A FUSION PROTEIN SUITABLE TO BE EXPRESSED HIGH EFFECTIVELY AND THE PRODUCTION METHOD THEREOF

摘要

The invention provides a novel fusion protein suitable to be expressed high effectively and the production method thereof. The structure form of the fusion protein is: A-Cl-K-(B-C2-K)n D, wherein A is the upstream peptide; C1 and C2 are each independently a short peptide of 20-40 amino acids of length and contain no Lys in sequence; K is Lys; B is linker; D is downstream peptide; n is integer of 3-30. The fusion protein is able to express high effectively and steadily in host cells. The purification and enzymic cut technology of the expression product is simple, which is able to produce a short peptide high effectively, and has very high value of industrialization.