| 摘要 |
The present invention is directed to a method for detection of a presence of one or more target molecules in a sample. This method involves providing a microdialysis unit having a membrane which defines within it a dialysate-containing region and contacting the microdialysis unit with a sample, potentially containing one or more target molecules, under conditions effective to permit molecules in the sample to permeate the membrane and enter the dialysate-containing region as a dialysate. A perfusate liquid comprising capture agents which are specific to the one or more target molecules and are immobilized to solid structures is provided and contacted with the dialysate (desirably within the dialysate-containing region) under conditions effective to permit target molecules present in the dialysate, if any, to specifically bind to the capture agents immobilized to the solid structures. As a result, a dialysate liquid potentially comprising dialysate complexes of target molecules bound to capture agents, which are immobilized to solid structures, is formed. The dialysate liquid is analyzed for dialysate complexes. As a result, the presence of the one or more target molecules in the sample is detected. A kit for carrying out this method is also disclosed.
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