Method for increasing the solubility, expression rate and the acitivity of proteins during recombinant production

摘要

The present invention concerns a method for producing a lysate containing helper proteins in which a strain which is suitable for obtaining in vitro translation lysates is transformed using a vector containing one or more genes coding for one or more helper proteins, wherein the helper proteins are expressed in this strain and the lysate containing helper proteins is obtained from these strains. The present invention also concerns a lysate containing helper proteins that can be obtained by the method according to the invention, blends of these lysates and the use of these lysates and blends in in vitro translation systems.