Culture systems for the sterile continuous cultivation of cells

摘要

The invention relates to culture systems and methods for continuous sterile cultivation of cells in high densities and for reducing the inoculation density at the beginning of cell cultivation in bioreactors. By using biodegradable gels that release low-molecular-weight constituents as nutrients for the cell culture or semi-solid media that are diluted during the course of the culturing and thus free up culture space for colonization with cells in high density, it is possible to sharply reduce the starting cell density at the beginning of cultivation in bioreactors. Biodegradable gels used are polypeptide (block) copolymers, in part comprising poly-L-glutamine, and semi-solid media used are methylcellulose, alginates, and agaroses.