| 发明名称 |
METHOD OF REVERSE TRANSCRIPTION USING BACILLUS STERAOTHERMOPHILUS OR THERMOACTINOMYCES VULGARIS DNA POLYMERASE |
| 摘要 |
The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided. |
| 申请公布号 |
EP1266033(A4) |
申请公布日期 |
2004.12.08 |
| 申请号 |
EP20010913200 |
申请日期 |
2001.02.28 |
| 申请人 |
PROMEGA CORPORATION |
发明人 |
GU, TRENT;HUANG, FEN;HARTNETT, JAMES, ROBERT |
| 分类号 |
C12N15/09;C12N9/12;C12N15/10;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/09 |
| 代理机构 |
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