DNA construct for use in filamentous fungi - comprising promoter operative in filamentous fungi to promote transcription of coding region

摘要

A novel DNA construct comprises a promoter operative in a filamentous fungus to promote transcription of a coding region when operatively associated. The promoter region naturally associated with the alcohol dehydrogeanse I (alcA) gene and the aldehyde dehydrogenase (aldA) gene of A. nidulans or naturally associated with the glucoamylase gene in Aspergillus niger may be used. The DNA construct may contain a promoter region in operative association with a signal peptide coding region and permits introduction of a region coding for a desired protein at a position 3' of and in reading frame with the signal peptide coding region. The promoter/signal construct is suitably provided with a flanking restriction site to allow precise coupling of the protein coding region to the signal peptide coding region.