PROCESS FOR PRODUCING EXTRACT FOR CELL-FREE PROTEIN SYNTHESIS AND CELL EXTRACT PRODUCED THEREBY

摘要

It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under regulated growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular, Escherichia coli cells. In the regulated growth conditions as described above, the culture temperature is preferably from 20 to 32°C, still preferably 26°C or higher and lower than 30°C.