| 摘要 |
Method and system for partitioning pixels of a region within a scanned, digital image of a microarray into a set of feature pixels and a set of background pixels based on a difference between the intensity variance or noise within a subregion of pixels corresponding to a feature and the variance of background pixels. In one embodiment of the present invention, a standard deviation and mean for the pixel intensities within a microarray-image region are computed, assuming a normal distribution for the noise, and are used to compute a one-dimensional computed probability distribution function for the pixels in the microarray-image region. The one-dimensional, computed probability distribution is generally bimodal, with pixels within a first peak at a lower-probability region of the computed probability distribution corresponding to feature pixels and pixels within a second, larger peak at a relatively larger computed probability corresponding to background pixels. A threshold between the first peak and second peak is used to threshold, or partition, the pixels of the microarray-image region into a set of feature pixels and a set of background pixels.
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