发明名称 |
Terminal-phosphate-labeled nucleotides with new linkers |
摘要 |
The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.
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申请公布号 |
US2004241716(A1) |
申请公布日期 |
2004.12.02 |
申请号 |
US20040772996 |
申请日期 |
2004.02.05 |
申请人 |
KUMAR SHIV;MCDOUGALL MARK;SOOD ANUP;NELSON JOHN;FULLER CARL;MACKLIN JOHN;MITSIS PAUL |
发明人 |
KUMAR SHIV;MCDOUGALL MARK;SOOD ANUP;NELSON JOHN;FULLER CARL;MACKLIN JOHN;MITSIS PAUL |
分类号 |
C12N;C12N1/00;C12N9/22;C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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