EXTERNAL GRADIENT CHROMATOFOCUSING

摘要

The invention provides novel methods for the separation of charged molecules such a proteins according to their isoelectric points (pI's) and includes th e systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pl's by shifting both the buffer's pKa and th e pl's of the eluted charged molecules while they are traversing the ion exchange column.