| 摘要 |
<p>Biological samples containing nucleic acids, RNA and DNA, are freed from bou nd proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from on e another or from other components of the reaction mixture. Methods include su ch preparation and further processing, such as amplification and detection, whi ch may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents.</p> |
