GLYCOSIDASES, NUCLEIC ACIDS ENCODING THEM AND METHODS OF MAKING AND USING THEM

摘要

The invention is directed to enzymes having a glycosidase activity, including thermostable and thermotolerant glycosidase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The glycosidase activities of the enzymes of the invention include the hydrolysis of any glycosidic linkages. For example, a glycosidase activity of an enzyme of the invention includes hydrolysis of a beta-anomeric configuration of a glycosidic linkage, such as the hydrolysis of beta-glucosides, beta-fucosides and beta-galactosides. Another glycosidase activity of an enzyme of the invention includes alpha-galactosidase activity, e.g., catalysis of the hydrolysis of galactose groups on a polysaccharide backbone or catalyzing the hydrolysis of the cleavage of di- or oligosaccharides comprising galactose. Another glycosidase activity of an enzyme of the invention includes catalysis of the hydrolysis of mannose groups internally on a polysaccharide backbone or hydrolysis of the cleavage of di-or oligosaccharides comprising mannose groups. Beta-mannosidases of the invention can hydrolyze non-reducing, terminal mannose residues on a mannose-containing polysaccharide and catalyze the cleavage of di- or oligosaccharides comprising mannose groups.