COMPOSITIONS AND METHODS FOR SITE-SPECIFIC RECOMBINATION AND EXPRESSION

摘要

The present invention provides materials and methods for the in vitro transcription and translation of nucleic acid sequence of interest. Methods may include a recombination reaction between a nucleic acid comprising a sequence of interest and an additional nucleic acid molecule comprising one or more recombination sites. The invention also includes kits for performing the methods of the invention. The invention also provides methods of conducting a business for providing nucleic acid molecules and polypeptides produced according to the methods of the invention.