发明名称 |
COMPOSITIONS AND METHODS FOR EVALUATING VIRAL RECEPTOR/CO-RECEPTOR USAGE AND INHIBITORS OF VIRUS ENTRY USING RECOMBINANT VIRUS ASSAYS |
摘要 |
The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell. |
申请公布号 |
EP1481098(A1) |
申请公布日期 |
2004.12.01 |
申请号 |
EP20030713444 |
申请日期 |
2003.02.14 |
申请人 |
VIROLOGIC, INC. |
发明人 |
RICHMAN, DOUGLAS;WRIN, MARY, T.;LITTLE, SUSAN;PETROPOULOS, CHRISTOS, J.;PARKIN, NEIL, T.;WHITCOMB, JEANNETTE, M.;HUANG, WEI |
分类号 |
C12N15/86;C12Q1/68;C12Q1/70 |
主分类号 |
C12N15/86 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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