发明名称 |
Methods and reagents for inactivating ribonucleases |
摘要 |
The present invention is a general method for inactivating or inhibiting ribonucleases. Ribonucleases are treated with a reducing agent and heat. RNA samples contaminated with ribonuclease may be treated with this method to protect them from degradation. The RNA may then be used directly in a variety of enzymatic reactions and molecular biology techniques. This method may also be applied to a variety of molecular biology reagents which may be contaminated with ribonuclease to protect an RNA from being degraded when incubated with the reagent.
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申请公布号 |
US6825340(B2) |
申请公布日期 |
2004.11.30 |
申请号 |
US20010815577 |
申请日期 |
2001.03.23 |
申请人 |
AMBION, INC. |
发明人 |
PASLOSKE BRITTAN L.;WU WILLIAM |
分类号 |
C07H1/08;C07H21/00;C07H21/02;C07H21/04;C12N;C12N1/08;C12N1/21;C12N9/12;C12N9/22;C12N9/99;C12P19/34;C12Q1/68;(IPC1-7):C07H21/02 |
主分类号 |
C07H1/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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