METHOD, REAGENT AND KIT FOR THE DETECTION OF FECAL OCCULT BLOOD

摘要

A fecal specimen may be tested for the presence of occult blood combining th e fecal specimen with: (a) an oxidizable substrate that produces a colored product in the presence of peroxide and hemoglobin; (b) hydrogen peroxide or a peroxide source; and (c) an enhancer selected to enhance the sensitivity of the test. Suitable enhancers are monocyclic nitrogen-containing aromatic heterocyclic compounds; tertiary or quaternary ammonium compounds having a phenyl, hydroxy alkyl or esterified hydroxy alkyl attached to the nitrogen; or quinoline or a substituted derivative thereof. If hemoglobin is present in the fecal sample, the oxidizable substrate is converted to the visibly detectable, colored product.