发明名称 |
Synthesis and recovery of aspartame involving enzymatic deformylation step |
摘要 |
The invention relates to the synthesis of aspartame involving enzymatic deformylation of an N-formyl-alpha-L-aspartyl-L-phenylalanine compound by treatment with an enzyme having formylmethionyl peptide deformylase activity and having as a co-factor group 5 to 11 bivalent metal ions. The invention also relates to selective preparation and recovery of aspartame from a mixture of N-formyl-alpha- and beta-L-aspartyl-L-phenylalanine compounds by treatment with such enzyme. And finally, the invention relates to one-pot enzymatic synthesis of aspartame from N-formyl-L-aspartic acid and L- or D,L-phenylalanine methyl ester involving an enzymatic deformylation reaction simultaneously with an enzymatic coupling reaction, as well as to one-pot di- or oligopeptide synthesis by simultaneous enzymatic coupling and deformylation reactions in general.
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申请公布号 |
US2004234944(A1) |
申请公布日期 |
2004.11.25 |
申请号 |
US20030624640 |
申请日期 |
2003.07.23 |
申请人 |
QUAEDFLIEG PETER J.L.M.;SONKE THEODORUS;WAGNER ADOLF F.V. |
发明人 |
QUAEDFLIEG PETER J.L.M.;SONKE THEODORUS;WAGNER ADOLF F.V. |
分类号 |
C07K5/072;C07K5/075;C12N9/80;C12P13/00;C12P41/00;(IPC1-7):C12Q1/00 |
主分类号 |
C07K5/072 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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