发明名称 |
In vitro translation system |
摘要 |
In vitro translation (IVT) systems and methods for increased expression of proteins from linear templates, using GamS, are provided. The proteins may be full length or protein fragments. The IVT system may be used in batch or continuous mode. The GamS may be used as GamS nucleic acid template, crude protein fraction, or purified protein product. The IVT system using GamS component may be employed in a high-throughput mode. The ability to predict expressible protein or fragments, and activity and solubility of a large-scale protein expression product based on the results obtained from high-throughput, small-scale IVT expression product is also provided.
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申请公布号 |
US2004235029(A1) |
申请公布日期 |
2004.11.25 |
申请号 |
US20040832820 |
申请日期 |
2004.04.27 |
申请人 |
LEE JAE MOON;BUCKLEY DOUGLAS IWEN;CANCILLA MICHAEL ROBERT;CURTIS DAMIAN E.;BOWMAN KRISTA K.;ZHAN HANGJUN;CIANCIO MARGIE |
发明人 |
LEE JAE MOON;BUCKLEY DOUGLAS IWEN;CANCILLA MICHAEL ROBERT;CURTIS DAMIAN E.;BOWMAN KRISTA K.;ZHAN HANGJUN;CIANCIO MARGIE |
分类号 |
C12N;C12N15/67;C12P21/00;C12P21/02;C12Q1/68;G01N33/53;(IPC1-7):C12Q1/68 |
主分类号 |
C12N |
代理机构 |
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代理人 |
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地址 |
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