发明名称 |
Method for producing a synthetic gene or other DNA sequence |
摘要 |
Disclosed herein is a method for synthesizing a desired nucleic acid sequence. The method comprises dividing the desired sequence into a plurality of partially overlapping segments; optimizing the melting temperatures of the overlapping regions of each segment to disfavor hybridization to the overlapping segments which are non-adjacent in the desired sequence; allowing the overlapping regions of single stranded segments which are adjacent to one another in the desired sequence to hybridize to one another under conditions which disfavor hybridization of non-adjacent segments; and filling in, ligating, or repairing the gaps between the overlapping regions, thereby forming a double-stranded DNA with the desired sequence. Also disclosed is a method for preventing errors in the synthesis of the nucleic acid sequence.
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申请公布号 |
US2004235035(A1) |
申请公布日期 |
2004.11.25 |
申请号 |
US20040851383 |
申请日期 |
2004.05.21 |
申请人 |
LATHROP RICHARD H.;HATFIELD G. WESLEY |
发明人 |
LATHROP RICHARD H.;HATFIELD G. WESLEY |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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