| 摘要 |
The invention relates to AAV helper plasmids for the helper virus-free packaging of AAV vectors. These AAV helper plasmids comprise the following DNA sequences: (a1) the rep gene of AAV-2, and (a2) the cap gene of AAV-1, AAV-3, AAV-4, AAV-5 or AAV-6, or (b) the cap gene and the rep gene of AAV-1, AAV-3, AAV-4, AAV-5 or AAV-6 each, and (c) all of the other helper virus DNA sequences necessary for forming AAV particles. The invention also relates to the use of these AAV helper plasmids or AAV particles with a coat encoded by these AAV helper plasmids and an AAV expression vector for gene therapy.
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