This disclosure describes three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed method has the advantage of not requiring tehrmocycling or thermophilic enzymes. Further, the improved processivity of the dislosed methods allow amplification of DNA up to hundreds of megabases in length.
申请公布号
WO03072805(A3)
申请公布日期
2004.11.25
申请号
WO2003US05492
申请日期
2003.02.21
申请人
ASM SCIENTIFIC, INC.;ARMES, NIALL, A.;STEMPLE, DEREK, L.