METHODS AND APPARATUS FOR ADENO ASSOCIATED VIRUS PURIFICATION

摘要

The present invention provides an apparatus for the purification of Adeno-associated virus (AAV) and methods of use including an anion exchange filter unit and a cation exchange capture unit. At one end, the cation exchange capture unit reversibly engages the anion exchange filter unit and when engaged is in fluid communication. The cation exchange capture unit may, at the opposing end, reversibly engage a syringe or apparatus able to provide negative pressure to draw a fluid containing AAV through the anion exchange filter unit then cation exchange capture unit where the AAV is captured. The anion exchange filter unit is disengaged and the purified AAV is eluted from the cation exchange capture unit.