| 摘要 |
A method is disclosed for identifying or analyzing, easily and in a short time, the molecular weight, the chemical species of molecules, and the structure of molecules, of a protein present on the superficial layer of cells or in cells, or a substance interacting with the protein. This method comprises the steps of: bringing the cells having the protein and the substance interacting with the protein into contact with each other to cause the substance to interact with the cells; purifying the cells, with the cells and the substance in an interacting state; irradiating the purified cells with a laser to ionize the substance directly; and analyzing the ionized substance by mass spectrometry. This method is a new method for screening a substance interacting with a protein present in cells, and a new method for determining the presence of a particular protein in cells with the use of a substance interacting with the protein, the new methods not relying on conventional bioassays. These methods are easy to operate and capable of accurate and prompt determination.
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