发明名称 |
Forbedret fremgangsmåde til bisulfitbehandling |
摘要 |
The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/ or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/ or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase. |
申请公布号 |
DK1394173(T3) |
申请公布日期 |
2008.02.04 |
申请号 |
DK20030019321T |
申请日期 |
2003.08.27 |
申请人 |
F.HOFFMANN-LA ROCHE AG;EPIGENOMICS AG |
发明人 |
MARKERT-HAHN, CHRISTINE, DR.;BLOCK, DIRK, DR. |
分类号 |
C07H21/00;C12N15/09;C12Q1/68 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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