A method of cleaving a target RNA molecule is disclosed. In one embodiment the method comprises the step of exposing the target molecule to an eukaryotic tRNA splicing endonuclease, wherein the target molecule is in the bulge-helix-bulge conformation, wherein cleavage occurs within the bulge-helix-bulge and cleavage products are generated, and wherein the target molecule does not comprise a tRNA structure.
申请公布号
WO2004090133(A3)
申请公布日期
2004.11.18
申请号
WO2004EP03915
申请日期
2004.04.14
申请人
ISTITUTO DI BIOLOGIA CELLULARE, CNR;TOCCHINI-VALENTINI, GLAUCO, P.;DEIDDA, GIANCARLO;ROSSI, NICOLETTA