The use of riluzol combined with excipients and additives for the treatment of disorders characterised by hyperproliferation of keratinocytes, in particular neurodermitis and psoriasis

摘要

In the production of a medicament for the prevention of a disease characterized by hyperproliferation of keratinocytes and/or T cells, Riluzole (I) or its salts, and optionally adjuvants and additives are used. Independent claims are included for: (1) a composition (C1) comprising (I) and active ingredients or calcineurin antagonists; and (2) topical medicament (M1) for transdermal delivery comprising (I) and a topical excipient. ACTIVITY : Cytostatic; Dermatological; Antipsoriatic; Keratolytic; Antiinflammatory; Immunosuppressive; Antilichen; Antiallergic; Antiseborrheic; Antiparkinsonian; Muscular-Gen.; Neuroprotective; Neuroleptic; Sedative; Antidepressant; Analgesic; Tranquilizer; Vulnerary; Cerebroprotective; Auditory; Relaxant; Anticonvulsant; Hypnotic. The effect of Riluzole on the proliferation of keratinocytes was examined on the basis of Ha-CaT cells as follows. 5 X 10 3>Ha-CaT keratinocytes were seeded into 60 wells of a 96 well-plate in KBM/10% FCS (200 Microl) and incubated for 24 hours at 37[deg]C. After incubation of 6 wells with Ha-CaT cells and 1 well without cells were treated for 48 hours with negative control (KMB/1% DMSO), positive control (KBM/FCS/1% DMSO) or with Riluzole (250 Micromol) in KBM/FCS (100 mmol/l in DMSO) and incubated for 48 hours at 37[deg]C. The concentration of DMSO was kept constant at 1% at all tested Riluzole concentrations. At the end of the second incubation period the medium was removed and the proliferation of cells was determined with the Cell Titer Viability Assay. The results showed that Riluzole completely inhibited proliferation of keratinocytes with values comparable with that achieved with the negative control. MECHANISM OF ACTION : Hyperproliferation of keratinocytes and/or T cells inhibitor.