发明名称 |
Column device for isolation and labeling of nucleic acids |
摘要 |
A method for manipulating genetic material, the method comprising disrupting cells so as to liberate genetic material contained in the cells; contacting the genetic material to a silica column in a manner to cause the genetic material to become immobilized to the column; labeling the immobilized genetic material; and eluting the labeled material from the column. Also provided is a two-buffer process for manipulating genetic material, the process comprising: contacting cells containing the genetic material to a silica column; creating a first fraction of cell detritus and a second fraction containing the genetic material; confining the genetic material to the column; removing the cell detritus; contacting the genetic material with radicals so as to produce reactive aldehyde groups on the genetic material, and attaching chromophore to the genetic material.
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申请公布号 |
US6818398(B2) |
申请公布日期 |
2004.11.16 |
申请号 |
US20000751654 |
申请日期 |
2000.12.29 |
申请人 |
THE UNIVERSITY OF CHICAGO |
发明人 |
BAVYKIN SERGEI G.;AKOWSKI JAMES P.;ZAKHARIEV VLADIMIR M.;MIRZABEKOV ANDREI |
分类号 |
B01D15/08;B01J20/283;C07H21/00;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68;C07H21/02 |
主分类号 |
B01D15/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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