| 主权项 |
1. A system for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation comprising:
(i) a laser source connected to a biological sample via excitation fibers (ExF), wherein the laser source is configured to radiate the biological sample with a laser pulse at a predetermined wavelength to cause the biological sample to produce a responsive fluorescence signal; (ii) collection fibers (CF), wherein the CF collect the fluorescence signal from the biological sample, and relays the fluorescence signal to a demultiplexer; (iii) a demultiplexer configured to split the fluorescence signal from the CF at pre-determined wavelengths to obtain spectral bands of <365 nm , 365-410 nm, 410- 450 nm, 450-500 nm, 500-560 nm, 560-600 nm and >600 nm,
wherein the demultiplexer comprises a first wavelength splitting device at about 500 nm, a second wavelength splitting device at about 560 nm, a third wavelength splitting device at about 600 nm, a fourth wavelength splitting device at about 410 nm, a fifth wavelength splitting device at about 450 nm, and a sixth wavelength splitting device at about 365 nm,wherein the fluorescence signal from the CF is split by the first wavelength splitting device into a <500 nm signal and a >500 nm signal, the >500 nm signal is split by the second wavelength splitting device into a 500-560 nm signal and a >560 nm signal, the >560 nm signal is split by the third wavelength splitting device into a 560-600 nm signal and a >600 nm signal, the <500 nm signal is split by the fourth wavelength splitting device into a <410 nm signal and a 410-500 nm signal, the 410-500 nm signal is split by the fifth wavelength splitting device into a 410-450 nm signal and a 450-500 nm signal, and the <410 nm signal is split by the sixth wavelength splitting device into a <365 nm signal and a 365-410 nm signal; and (iv) an optical delay device. |
